Thanks for the response. My apologies but after checking how Process Lasso was being launched, I was at fault.

I have been using Glarysoft Startup Manager to delay some of my startup programs and Process Lasso was one. I have removed it and it seems like the column layout is saved for the while.
I understand what you said about the ini file, no worries. I just thought it would have been a good solution and perhaps more permanent if one wants to backup or save settings.
Good to know about registry cleaners! I'll be sure to look out for it in CCleaner...if I remember.
This is probably off-topic but is it fine to launch Process Lasso using this?
"C:\Program Files\Process Lasso\ProcessLassoLauncher.exe" /nodelay